Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

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The 1970s brought about many developments in hardware and instrumentation. Researchers began using pumps and injectors to make a rudimentary design of an HPLC system. [6] Gas amplifier pumps were ideal because they operated at constant pressure and did not require leak free seals or check valves for steady flow and good quantitation. [4] Hardware milestones were made at Dupont IPD (Industrial Polymers Division) such as a low-dwell-volume gradient device being utilized as well as replacing the septum injector with a loop injection valve. [4]

Gas chromatography is based on a partition equilibrium of analyte between a solid or viscous liquid stationary phase (often a liquid silicone-based material) and a mobile gas (most often helium). The stationary phase is adhered to the inside of a small-diameter (commonly – inside diameter) glass or fused-silica tube (a capillary column) or a solid matrix inside a larger metal tube (a packed column). It is widely used in analytical chemistry ; though the high temperatures used in GC make it unsuitable for high molecular weight biopolymers or proteins (heat denatures them), frequently encountered in biochemistry , it is well suited for use in the petrochemical , environmental monitoring and remediation , and industrial chemical fields. It is also used extensively in chemistry research.

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Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

But having defined height will help avoid layout shifting depending on the amount of content that is inside the carousel

  • Superior analytical performance from our state-of-the-art instruments for sample collection, punching and analysis and measurement.
  • Earlier detection of NBS disorders with our complete range of screening assays
  • Increased process throughput and reduced costs from sample to result with our dedicated Informatics solutions for newborn screening laboratories
For comprehensive information about our products, technologies and software for newborn screening, please visit our newborn screening site.

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